Currently, for HLVd testing, our lab utilizes RT-PCR technology with a host control gene. This ensures that there are no false negatives due to sample degradation or a failed PCR/LAMP reaction as the host control gene is detected in every reaction. Furthermore, RT-PCR is much less likely to produce false positives compared to its counterpart RT-LAMP which uses enzymes possessing a higher rate of off-target amplification. Our RT-PCR also has an additional layer of testing integrity as two separate targets of HLVd are detected at the same time, something that RT-LAMP is not capable of.